PI组长：
       施春雷  研究员    

组员：
       张建华  

研究方向：

食源性致病菌致病和抗药性机理

主要研究领域：

1、食源性致病菌快速检测分型与风险评估

2、食源性致病菌菌膜形成分子机制

3、食源性致病菌耐药形成传播机制

4、食品微生物菌种改良及生物酶的高效利用

PI研究组代表性成果：

1、主要研究项目：

1）2020年1月-2023年12月，萘替芬衍生物对金黄色葡萄球菌黄素合成和生物膜形成的双效抑制机制，国家自然基金面上项目，31972169，58万元，施春雷为项目主持人

2）2018年1月-2021年12月，重要食源性致病菌耐药机制及传播规律研究，国家重点研发计划“食品安全关键技术研发”专项，2017YFC1600100，2980万元，施春雷为项目主持人

3) 2018年12月1日-2021年12月31日，国家重点研发计划子课题 食品监管微生物追踪技术与网络平台的建立，2018YFC1603901-5，71.4/429/2431万元，张建华为子课题主持人

4）2017年1月-2020年12月，葡萄球菌黄素合成与菌膜形成的相关性及其分子调控机制，国家自然基金面上项目，31671943，63万元，施春雷为项目主持人

5）2016年7月-2020年12月，跨境食品潜在、新发病原微生物筛查监控技术及溯源平台建设，国家重点研发计划项目“跨境食品品质与质量控制数据库构建及创新集成开发”，2016YFD0401102，88/494/2600万元，施春雷为子课题主持人

2、成果专利与奖励：

1) 施春雷, 庄孝飞, 周秀娟, 史贤明, 崔妍. 一种沙门氏菌血清型鉴定方法及其试剂盒, ZL201410293102.7

2) 施春雷, 杨捷琳, 史贤明, 刘斌, 潘良文, 袁辰刚. 一种蜡样芽孢杆菌的检测方法及试剂盒, ZL201410253239.X

3) 施春雷, 杨捷琳, 史贤明, 刘斌, 潘良文, 袁辰刚. 蜡样芽孢杆菌的检测方法及试剂盒, ZL201210308753.X

4) 张建华, 刘亚楠, 钱炳俊 , 姚晓敏 , 亚力坤江·阿山, 王延辉. 一种抗氧化肽混合物及其制备方法, ZL201610303716.8

5) 张建华，尹丽茸，倪银芸，左琦，李双双，林立. 一种混合菌株发酵生产茶籽粕饲料的方法, ZL201310014799.5

3、代表论著：

1) Zhao H, Chen WY†, Xu XB, Zhou XJ, Shi CL*. 2018. Transmissible ST3-IncHI2 plasmids are predominant carriers of diverse complex IS26-class 1 integron arrangements in multidrug-resistant Salmonella. Frontiers in Microbiology, 9: 2492.

2) Liu AL, Shi CL*. 2018. Pork juice promotes biofilm formation in Listeria monocytogenes. Journal of Food Safety, 38(2): e12439.

3) Zhang J, Suo YJ†, Zhang DF, Jin FN, Zhao H, Shi CL*. 2018. Genetic and virulent differences between pigmented and non-pigmented Staphylococcus aureus. Frontiers in Microbiology, 9: 598.

4) Huang YY, Morvay AA†, Shi XM, Suo YJ, Shi CL*, Knøchel S*. 2018. Comparison of oxidative stress response and biofilm formation of Listeria monocytogenes serotypes 4b and 1/2a. Food Control, 85: 416-422.

5) Song MH, Li QQ, Zhang Y, Song JX, Shi XM, Shi CL*. 2017. Biofilm formation capacity and antibiotic resistance pattern of dominant Staphylococcus aureus clonal lineages in China. Journal of Food Safety, 37(2): e12304.

6) Chen WY, Fang TZ†, Zhou XJ, Zhang DF, Shi XM, Shi CL*. 2016. IncHI2 plasmids are predominant in antibiotic-resistant Salmonella isolates. Frontiers in Microbiology, 7: 1566.

7) Yang JL, He YP, Jiang J, Chen WY, Gao Q, Pan LW, Shi CL*. 2016. Comparative proteomic analysis by iTRAQ-2DLC-MS/MS provides insight into the key proteins involved in Cronobacter sp. biofilm formation. Food Control, 63:93-100.

8) Shi CL, Singh P, Ranieri ML, Wiedmann M, Switt AM*. 2015. Molecular methods for serovar determination of Salmonella. Critical Reviews in Microbiology, 41(3): 309-325.

9) Li MY, Cui Y, Gan ZB, Shi CL*, Shi XM. 2015. Isolation and analysis of the Cppsy gene and promoter from Chlorella protothecoides CS-41. Marine Drugs, 13(11): 6620-6635.

10) Zhou XJ, He SK, Song QF, Zhuang XF, Wang YY, Huang XZ, Shi CL*, Shi XM*. 2015. Phenotypic and genotypic characterization of Salmonella enterica serovar Enteritidis isolates associated with a mousse cake-related outbreak of gastroenteritis in Ningbo China. Foodborne Pathogens and Disease, 12(5): 433-440.

11) Song MH, Bai YL, Xu J, Carter MQ, Shi CL*, Shi XM*. 2015. Genetic diversity and virulence potential of Staphylococcus aureus isolates from raw and processed food commodities in Shanghai. International Journal of Food Microbiology, 195: 1-8.

12) Zhou XJ, Liu B, Shi CL*, Shi XM*. 2014. Mutation of a Salmonella serogroup-C1-specific gene abrogates O7-antigen biosynthesis and triggers NaCl-dependent motility deficiency. PLoS One, 9(9): e106708.

13) Xu J, Shi CL†, Song M, Xu X, Yang P, Paoli G, Shi XM*. 2014. Phenotypic and genotypic antimicrobial resistance traits of foodborne Staphylococcus aureus isolates from Shanghai. Journal of Food Science, 79(4): M635-642.

14) Li MY, Gan ZB, Cui Y, Shi CL*, Shi XM*. 2013. Structure and function characterization of the phytoene desaturase related to the lutein biosynthesis in Chlorella protothecoides CS-41. Molecular Biology Reports, 40 (4): 3351-3361.

15) Li MY, Gan ZB, Cui Y, Shi CL*, Shi XM*. 2011. Cloning and characterization of the ζ-carotene desaturase gene from a unicellular microalga, Chlorella protothecoides CS-41. Journal of Biomedicine and Biotechnology (now Biomed Research International), Volume 2011, Article ID 731542, 7 pages.

16) Tang HQ, Qian BJ, Xia B, Zhuan Y, Yao YQ, Gan RY, Zhang JH*. 2018. Screening of lactic acid bacteria isolated from fermented Cornus officinalis fruits for probiotic potential. Journal of Food Safety, 38(6):e12565.

17) Qian BJ, Yin LR, Yao XM, Zhong YG, Gui J, Lu FF, Zhang FM and Zhang JH*. 2018. Effects of fermentation on the hemolytic activity and degradation of Camellia oleifera saponins by Lactobacillus crustorum and Bacillus subtilis. FEMS Microbiology Letters, 365(7): fny014.

18) Wu BY, Qian BJ, Zhu Q, Shi CL, Li LY, Yao XM, Zhang JH *. 2017. Purification and identification of an angiotensin I-converting enzyme-inhibitory peptide from Argopecten irradians mantle enzymatic hydrolysate. The Journal of European Food Research and Technology. 243:711-717.

19) Zhang FM, Zhang JH *, Linhardt RJ*. 2015. Interactions between nattokinase and heparin/GAGs. Glycoconjugate Journal. 32:695-702.

20) Zhang JH*, Suflita M, Li GY, Zhong WH, Li LY, Dordick JS, Linhardt RJ, Zhang FM *. 2015. High cell density cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase for the production of bioengineering heparin. Applied Biochemistry and Biotechnology, 175: 2986-2995.

21) Zhang JH*, Suflita M, Li GY, Li LY, Zhang FM, Dordick JS, Linhardt RJ *. 2015. High cell density cultivation of a recombinant Escherichia coli strain expressing a 6-O-sulfotransferase for the production of bioengineered heparin. Journal of Applied Microbiology, 118(1), 92-98.

22) Ni YY, Wang JL, Qian BJ, Song GY, Yao XM, Zhang JH *. 2014. Purification and side chain selective chemical modifications of glutamate dehydrogenase from Bacillus subtilis natto. Applied Biochemistry and Biotechnology, 172:3593-3605.

23) Chen LL, Wang JL, Hu Y, Qian BJ, Yao XM, Wang JF, Zhang JH *. 2013. Computational structure-based redesign of glutamate dehydrogenase in Bacillus subtilis natto. Journal of Molecular Modeling, 19(4):1919-1927.